| Insertion cassette: | CIB1 |
| Side of cassette: | 3' |
| Strand: | + |
| Strain: | LMJ.RY0402.080083 |
| Chromosome: | chromosome 16 |
| Location: | 1360375 |
| Confidence (%): | 58 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre16.g651900 | CRI1,OPR129,OPR126,CRTISO | OctotricoPeptide Repeat protein 129 | CDS |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | CCCGCAGCAATTAAGTAGCCTCACGTCAGT |
| Internal bar code: | TAAGAGGCGTCGCGTGCGTCGC |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 50 |
| LEAP-Seq percent confirming: | 97.4425 |
| LEAP-Seq n confirming: | 381 |
| LEAP-Seq n nonconfirming: | 10 |
| LEAP-Seq n unique pos: | 2 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | GCTTAACAGGGGTGTTGCAT |
| Suggested primer 2: | ACTTCGGTCACCTCCAACAC |