| Insertion cassette: | CIB1 |
| Side of cassette: | 3' |
| Strand: | + |
| Strain: | LMJ.RY0402.080187 |
| Chromosome: | chromosome 12 |
| Location: | 1893409 |
| Confidence (%): | 95 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre12.g511150 | GPD1 | (1 of 1) PTHR11728:SF13 - GLYCEROL-3-PHOSPHATE DEHYDROGENASE [NAD(+)] 2, CHLOROPLASTIC; Glycerol-3-phosphate dehydrogenase/dihydroxyacetone-3-phosphate reductase | 3'UTR |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | ATCCTGCCACAGTGCGGAGATACTTGCACG |
| Internal bar code: | AAGCCCCATTGCGGCCGGTTTA |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 741 |
| LEAP-Seq percent confirming: | 99.1552 |
| LEAP-Seq n confirming: | 2230 |
| LEAP-Seq n nonconfirming: | 19 |
| LEAP-Seq n unique pos: | 17 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | ATGAACACACCGTGCCAGTA |
| Suggested primer 2: | GCCTACTCGCTGATACTCGG |