| Insertion cassette: | CIB1 |
| Side of cassette: | 5' |
| Strand: | + |
| Strain: | LMJ.RY0402.080189 |
| Chromosome: | chromosome 17 |
| Location: | 5450783 |
| Confidence (%): | 73 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre17.g737084 | (1 of 1) PTHR15863//PTHR15863:SF2 - FAMILY NOT NAMED // UPF0544 PROTEIN C5ORF45 | CDS |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | TTTGTGGAGGATGAGGGCGGGGCGGATTGG |
| Internal bar code: | GGGTCCATTGTATTAATCAAGA |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 855 |
| LEAP-Seq percent confirming: | 99.7184 |
| LEAP-Seq n confirming: | 10977 |
| LEAP-Seq n nonconfirming: | 31 |
| LEAP-Seq n unique pos: | 11 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | CTGCTGTGTCAGGTGCTGAT |
| Suggested primer 2: | GGAGTGTCCATGTCCTTCGT |