| Insertion cassette: | CIB1 |
| Side of cassette: | 5' |
| Strand: | + |
| Strain: | LMJ.RY0402.080195 |
| Chromosome: | chromosome 17 |
| Location: | 5070050 |
| Confidence (%): | 95 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre17.g734805 | (1 of 8) PF00560 - Leucine Rich Repeat (LRR_1) | 3'UTR |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | CTGTGTTTACCACAGGCACCCCTGAGTCGC |
| Internal bar code: | TCTACTCCTCAGAGCCCGGAGA |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 1268 |
| LEAP-Seq percent confirming: | 98.8506 |
| LEAP-Seq n confirming: | 172 |
| LEAP-Seq n nonconfirming: | 2 |
| LEAP-Seq n unique pos: | 3 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | CGTCTGACGCACCACTAAGA |
| Suggested primer 2: | AAAACGTTCACCAATGGCTC |