Insertion cassette: | CIB1 |
Side of cassette: | 5' |
Strand: | - |
Strain: | LMJ.RY0402.080201 |
Chromosome: | chromosome_2 |
Location: | 5625951 |
Confidence (%): | 73 |
Locus systematic id | Locus common name | Defline | Orientation | Feature |
---|---|---|---|---|
Cre02.g109333 | sense | 3'UTR |
Insertion site details | |
Flanking sequence (orientation from cassette outwards): | GTTTCATTCGTCCGGCAATCCGCTTGCAAC |
Internal bar code: | TATCGTCCCCGGATTCGTACTC |
Confirmation - LEAP-Seq | |
LEAP-Seq distance: | 788 |
LEAP-Seq percent confirming: | 98.7873 |
LEAP-Seq n confirming: | 1059 |
LEAP-Seq n nonconfirming: | 13 |
LEAP-Seq n unique pos: | 3 |
Suggested primers for confirmation by PCR | |
Suggested primer 1: | GAGTGTGTGTTGGGCATTTG |
Suggested primer 2: | CTGGTGTTCTCCAGGTTGGT |