| Insertion cassette: | CIB1 |
| Side of cassette: | 3' |
| Strand: | - |
| Strain: | LMJ.RY0402.080211 |
| Chromosome: | chromosome 4 |
| Location: | 1186941 |
| Confidence (%): | 95 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre04.g215600 | (1 of 1) PTHR32183:SF6 - THIOCYANATE METHYLTRANSFERASE 1-RELATED | CDS |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | CTCGAAGCCTGCAGCGGAGCGTGAAATCCG |
| Internal bar code: | GAGGCAAACCGCGGAGGGGGAC |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 643 |
| LEAP-Seq percent confirming: | 98.784 |
| LEAP-Seq n confirming: | 2437 |
| LEAP-Seq n nonconfirming: | 30 |
| LEAP-Seq n unique pos: | 20 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | TGGCCGAATTCCTTAGATTG |
| Suggested primer 2: | GCGACTTCTTCAAGTTTGCC |