| Insertion cassette: | CIB1 |
| Side of cassette: | 3' |
| Strand: | - |
| Strain: | LMJ.RY0402.080225 |
| Chromosome: | chromosome 10 |
| Location: | 4251593 |
| Confidence (%): | 73 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre10.g450400 | NUOE,NUO5 | (1 of 1) K03943 - NADH dehydrogenase (ubiquinone) flavoprotein 2 (NDUFV2); NADH:ubiquinone oxidoreductase 24 kDa subunit | 3'UTR |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | GCATTAGTTCCGCAACGCACGTCGACAACG |
| Internal bar code: | CCGGGGACCTGAATGATTGCCG |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 534 |
| LEAP-Seq percent confirming: | 99.324 |
| LEAP-Seq n confirming: | 1910 |
| LEAP-Seq n nonconfirming: | 13 |
| LEAP-Seq n unique pos: | 7 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | GTGGAAGATATGGCAAGCGT |
| Suggested primer 2: | TTCTCGAACTGGTCTGGCTT |