| Insertion cassette: | CIB1 |
| Side of cassette: | 5' |
| Strand: | + |
| Strain: | LMJ.RY0402.080235 |
| Chromosome: | chromosome 12 |
| Location: | 226911 |
| Confidence (%): | 73 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre12.g485150 | GAP1a,GAP1,GAPC1 | (1 of 2) 1.2.1.12 - Glyceraldehyde-3-phosphate dehydrogenase (phosphorylating) / Triosephosphate dehydrogenase; Glyceraldehyde 3-phosphate dehydrogenase, GAPC, chloroplastic | 3'UTR |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | GTTCATTGGAGTGTGGAGCTGTATTGGCGC |
| Internal bar code: | CAGCTGGCCGTCCTGGATGAGT |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 662 |
| LEAP-Seq percent confirming: | 99.6103 |
| LEAP-Seq n confirming: | 7412 |
| LEAP-Seq n nonconfirming: | 29 |
| LEAP-Seq n unique pos: | 6 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | GCTGTACAACGCCTTCTTCC |
| Suggested primer 2: | GTGGTGTCCTCCGACTTTGT |