| Insertion cassette: | CIB1 |
| Side of cassette: | 3' |
| Strand: | - |
| Strain: | LMJ.RY0402.080237 |
| Chromosome: | chromosome 12 |
| Location: | 8634486 |
| Confidence (%): | 58 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre12.g546900 | (1 of 24) IPR006553 - Leucine-rich repeat, cysteine-containing subtype | intron |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | CGTGGCACTGCCGCTCAGCGCCGCGCACCT |
| Internal bar code: | TGGCTCTAGGGGTAAAGGGGAT |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 286 |
| LEAP-Seq percent confirming: | 72.5541 |
| LEAP-Seq n confirming: | 838 |
| LEAP-Seq n nonconfirming: | 317 |
| LEAP-Seq n unique pos: | 11 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | GAGATGCTATGGGGTCCTGA |
| Suggested primer 2: | TCCACTCCATCTCATCTCCC |