Insertion junction: LMJ.RY0402.080252_2

Overview

Insertion cassette:CIB1
Side of cassette:5'
Strand:-
Strain:LMJ.RY0402.080252
Chromosome:chromosome 9
Location:2109230
Confidence (%):95
Locus systematic id Locus common name Defline Feature
Cre09.g393700 MMP3 Matrix metalloproteinase; (1 of 2) IPR016517 - Peptidase M11, autolysin CDS

Insertion site details

Expected flanking sequence (starting at cassette; orientation from cassette outwards): TCCAGTCCAAGACCAGTACCTGGACGTCGC
Internal bar code:TGCCGTGGCTTGGATCGGTCCA

Confirmation - LEAP-Seq

LEAP-Seq distance:617
LEAP-Seq percent confirming:98.3101
LEAP-Seq n confirming:989
LEAP-Seq n nonconfirming:17
LEAP-Seq n unique pos:3

Suggested primers for confirmation by PCR

Suggested primer 1:CACCGACTTCGTTAAACGGT
Suggested primer 2:CACAACTACGGACTGGAGCA