| Insertion cassette: | CIB1 |
| Side of cassette: | 5' |
| Strand: | + |
| Strain: | LMJ.RY0402.080255 |
| Chromosome: | chromosome 1 |
| Location: | 273268 |
| Confidence (%): | 95 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre01.g001678 | (1 of 7) PTHR23257//PTHR23257:SF474 - SERINE-THREONINE PROTEIN KINASE // SUBFAMILY NOT NAMED | intron |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | CTCCCCATAGCCCCACCTTCTCCCCCCCTT |
| Internal bar code: | CACGACCGCTCAGACCGTCATG |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 1005 |
| LEAP-Seq percent confirming: | 64.4944 |
| LEAP-Seq n confirming: | 287 |
| LEAP-Seq n nonconfirming: | 158 |
| LEAP-Seq n unique pos: | 4 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | CCTTGCGTTTTCTTCTCTCG |
| Suggested primer 2: | ACTACACGTCCCACTACCCG |