| Insertion cassette: | CIB1 |
| Side of cassette: | 5' |
| Strand: | - |
| Strain: | LMJ.RY0402.080266 |
| Chromosome: | chromosome 5 |
| Location: | 2128489 |
| Confidence (%): | 95 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre05.g234640 | (1 of 1) IPR000048//IPR011989//IPR016024 - IQ motif, EF-hand binding site // Armadillo-like helical // Armadillo-type fold | intron |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | GTTTCCAGCCCAAATTCAATCAACCAAGCT |
| Internal bar code: | CCGCGTGTATTTAAGCGGCTTT |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 154 |
| LEAP-Seq percent confirming: | 100.0 |
| LEAP-Seq n confirming: | 88 |
| LEAP-Seq n nonconfirming: | 0 |
| LEAP-Seq n unique pos: | 4 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | CTCCTTGAACCCCTTGAACA |
| Suggested primer 2: | AATGCCAGTCCAACCTGAAC |