Insertion junction: LMJ.RY0402.080277_1

Overview

Insertion cassette:CIB1
Side of cassette:3'
Strand:-
Strain:LMJ.RY0402.080277
Chromosome:chromosome 2
Location:2717151
Confidence (%):73
Locus systematic id Locus common name Defline Feature
Cre02.g093600 TEH3 (1 of 6) PF03061 - Thioesterase superfamily (4HBT); Thioesterase-like protein 3'UTR

Insertion site details

Expected flanking sequence (starting at cassette; orientation from cassette outwards): TTAGAACGTACACAGCTGCCTTTTGTGATT
Internal bar code:TCGAAGCGACTTTGATATGAGT

Confirmation - LEAP-Seq

LEAP-Seq distance:561
LEAP-Seq percent confirming:98.8098
LEAP-Seq n confirming:4898
LEAP-Seq n nonconfirming:59
LEAP-Seq n unique pos:29

Suggested primers for confirmation by PCR

Suggested primer 1:CTGTTTGTAACGCCTCGTCA
Suggested primer 2:CACAGGTCGCTAACCAGACA