| Insertion cassette: | CIB1 |
| Side of cassette: | 3' |
| Strand: | + |
| Strain: | LMJ.RY0402.080278 |
| Chromosome: | chromosome 1 |
| Location: | 6874544 |
| Confidence (%): | 58 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre01.g049750 | (1 of 1) PTHR12714:SF11 - C-TERMINAL S-ISOPRENYLCYSTEINE CARBOXYL O-METHYLTRANSFERASE | CDS |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | GGAACCATCGCGCGCATGGTCAAGCACGGC |
| Internal bar code: | GGATTTTGCTGCAGGCGGTAAC |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 360 |
| LEAP-Seq percent confirming: | 41.8966 |
| LEAP-Seq n confirming: | 243 |
| LEAP-Seq n nonconfirming: | 337 |
| LEAP-Seq n unique pos: | 4 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | GTCACGACCCTGGACAAAGT |
| Suggested primer 2: | GAATACTGCTGCCTTTTCGC |