| Insertion cassette: | CIB1 |
| Side of cassette: | 3' |
| Strand: | + |
| Strain: | LMJ.RY0402.080307 |
| Chromosome: | chromosome 9 |
| Location: | 226866 |
| Confidence (%): | 95 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre09.g386755 | 3'UTR |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | CCGTCGAAACCGTGTCCAACAAAGTCTTGC |
| Internal bar code: | GCGTAGGCAAGGGTGCCGCAGA |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 585 |
| LEAP-Seq percent confirming: | 98.777 |
| LEAP-Seq n confirming: | 1050 |
| LEAP-Seq n nonconfirming: | 13 |
| LEAP-Seq n unique pos: | 8 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | CTAGAGGCAACCGCAGAGTC |
| Suggested primer 2: | ATGTTGTCGTAGGAGACGGG |