| Insertion cassette: | CIB1 |
| Side of cassette: | 5' |
| Strand: | - |
| Strain: | LMJ.RY0402.080309 |
| Chromosome: | chromosome 1 |
| Location: | 7230708 |
| Confidence (%): | 73 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre01.g052000 | ZNG1 | (1 of 1) PTHR13748//PTHR13748:SF31 - COBW-RELATED // COBW DOMAIN-CONTAINING PROTEIN 1; Zn chaperone | 3'UTR |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | CTGGTTGGCTTCGGTTCACGCTCTGCCCTT |
| Internal bar code: | TAATATTCGGCATATGTTATCA |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 442 |
| LEAP-Seq percent confirming: | 99.6791 |
| LEAP-Seq n confirming: | 10562 |
| LEAP-Seq n nonconfirming: | 34 |
| LEAP-Seq n unique pos: | 3 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | ACCTCAGAAACCTCGCTCAA |
| Suggested primer 2: | ATGTCGATTCACCCAAGAGC |