Insertion junction: LMJ.RY0402.080311_4

Overview

Insertion cassette:CIB1
Side of cassette:5'
Strand:-
Strain:LMJ.RY0402.080311
Chromosome:chromosome 9
Location:2259793
Confidence (%):95
Locus systematic id Locus common name Defline Feature
Cre09.g392652 (1 of 1) 1.5.1.22 - Strombine dehydrogenase 3'UTR

Insertion site details

Expected flanking sequence (starting at cassette; orientation from cassette outwards): GATGAAAGTTTGGATGAGTGCCGTAGGGAC
Internal bar code:GTCCCGGCAGTGAATATCGTC

Confirmation - LEAP-Seq

LEAP-Seq distance:843
LEAP-Seq percent confirming:99.6826
LEAP-Seq n confirming:7852
LEAP-Seq n nonconfirming:25
LEAP-Seq n unique pos:3

Suggested primers for confirmation by PCR

Suggested primer 1:GCGTGATGAAGAGGAAGAGG
Suggested primer 2:CTGATCAGTAGGGCTTTCGC