| Insertion cassette: | CIB1 |
| Side of cassette: | 5' |
| Strand: | + |
| Strain: | LMJ.RY0402.080324 |
| Chromosome: | chromosome 2 |
| Location: | 3390775 |
| Confidence (%): | 95 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre02.g095103 | GT90-34,GT90F34 | GT90 family protein 34; (1 of 52) PF05686 - Glycosyl transferase family 90 (Glyco_transf_90) | intron |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | GATGGCCAACTGCCCCCCTTGTACATGGAT |
| Internal bar code: | GCCAGTCCGCAGCTGTTAGGCA |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 705 |
| LEAP-Seq percent confirming: | 99.47 |
| LEAP-Seq n confirming: | 1126 |
| LEAP-Seq n nonconfirming: | 6 |
| LEAP-Seq n unique pos: | 5 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | CTGACACTCCCCTCCACAAT |
| Suggested primer 2: | CGTAAAGGCCAGACTTCGAG |