| Insertion cassette: | CIB1 |
| Side of cassette: | 3' |
| Strand: | + |
| Strain: | LMJ.RY0402.080352 |
| Chromosome: | chromosome 12 |
| Location: | 1761251 |
| Confidence (%): | 73 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre12.g512400 | (1 of 1) PTHR30224 - ELECTRON TRANSPORT PROTEIN | CDS/intron |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | GTGAGCCTTCTGTGCGGTGCTGCATGACGT |
| Internal bar code: | CCACAGTGGGTCGTCAGCAGGG |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 645 |
| LEAP-Seq percent confirming: | 96.2801 |
| LEAP-Seq n confirming: | 1760 |
| LEAP-Seq n nonconfirming: | 68 |
| LEAP-Seq n unique pos: | 14 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | CTGGTCTTTAGCCTTCTGCG |
| Suggested primer 2: | GGTGGGATCGAGCTCTACAA |