| Insertion cassette: | CIB1 |
| Side of cassette: | 5' |
| Strand: | - |
| Strain: | LMJ.RY0402.080379 |
| Chromosome: | chromosome 12 |
| Location: | 2729817 |
| Confidence (%): | 95 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre12.g503450 | (1 of 52) PF00856 - SET domain (SET) | 5'UTR |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | TGCAAAATGCGCGGAGGTTGCGCGCTAGTA |
| Internal bar code: | TTGCTAGCCCGAACTCGCTATG |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 669 |
| LEAP-Seq percent confirming: | 99.3658 |
| LEAP-Seq n confirming: | 470 |
| LEAP-Seq n nonconfirming: | 3 |
| LEAP-Seq n unique pos: | 3 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | AAGTGCATCTTCCCAACCAG |
| Suggested primer 2: | GGTCGAAAAGACTGCGAAAG |