| Insertion cassette: | CIB1 |
| Side of cassette: | 5' |
| Strand: | + |
| Strain: | LMJ.RY0402.080382 |
| Chromosome: | chromosome 2 |
| Location: | 471749 |
| Confidence (%): | 58 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre02.g076625 | intron |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | CGTACACGAATTAGTTGGGGGGTAGTCGGA |
| Internal bar code: | TCGGTTTCCTTCAACTGGTAGT |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 27 |
| LEAP-Seq percent confirming: | 90.9091 |
| LEAP-Seq n confirming: | 250 |
| LEAP-Seq n nonconfirming: | 25 |
| LEAP-Seq n unique pos: | 3 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | ACGGAATCACAACTTCCAGG |
| Suggested primer 2: | CCGCTTCCACACTTTCAAAT |