Insertion junction: LMJ.RY0402.080401_2

Overview

Insertion cassette:CIB1
Side of cassette:3'
Strand:+
Strain:LMJ.RY0402.080401
Chromosome:chromosome 13
Location:2585208
Confidence (%):95
Locus systematic id Locus common name Defline Feature
Cre13.g581050 GT90F46,GT90-46 (1 of 11) 2.4.2.26 - Protein xylosyltransferase / Uridine diphosphoxylose-protein xylosyltransferase; GT90 family protein 46 intron

Insertion site details

Expected flanking sequence (starting at cassette; orientation from cassette outwards): TTGTGATGCAAGACGCTTCCATGCGGAACT
Internal bar code:ATCAGGTGTTCTCAGAAAGATC

Confirmation - LEAP-Seq

LEAP-Seq distance:557
LEAP-Seq percent confirming:95.1969
LEAP-Seq n confirming:4400
LEAP-Seq n nonconfirming:222
LEAP-Seq n unique pos:21

Suggested primers for confirmation by PCR

Suggested primer 1:GGTTGTCGTCATAGGGCAGT
Suggested primer 2:CACGTAGTGCACATTGGGTC