Insertion junction: LMJ.RY0402.080402_2

Overview

Insertion cassette:CIB1
Side of cassette:5'
Strand:-
Strain:LMJ.RY0402.080402
Chromosome:chromosome 12
Location:7326741
Confidence (%):95
Locus systematic id Locus common name Defline Feature
Cre12.g557350 FAO9 (1 of 1) PF00355//PF01266 - Rieske [2Fe-2S] domain (Rieske) // FAD dependent oxidoreductase (DAO); FAD-dependent oxidoreductase intron

Insertion site details

Expected flanking sequence (starting at cassette; orientation from cassette outwards): TTGGCCATCCTCCCGGACTACTGTACGGTC
Internal bar code:GCTGGGCCTAGCCCGGCTCTAA

Confirmation - LEAP-Seq

LEAP-Seq distance:162
LEAP-Seq percent confirming:8.85287
LEAP-Seq n confirming:71
LEAP-Seq n nonconfirming:731
LEAP-Seq n unique pos:2

Suggested primers for confirmation by PCR

Suggested primer 1:ATCCCCGAACTCCTCTCTGT
Suggested primer 2:TCCTCCACAAAATGCTACCC