| Insertion cassette: | CIB1 |
| Side of cassette: | 3' |
| Strand: | + |
| Strain: | LMJ.RY0402.080424 |
| Chromosome: | chromosome 5 |
| Location: | 3027860 |
| Confidence (%): | 95 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre05.g238550 | 3'UTR |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | TGACCGGTTGTGCCATGTCGGACTAGTATT |
| Internal bar code: | CCTTGTCCGGTGGGAAACACTT |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 590 |
| LEAP-Seq percent confirming: | 99.2551 |
| LEAP-Seq n confirming: | 5063 |
| LEAP-Seq n nonconfirming: | 38 |
| LEAP-Seq n unique pos: | 27 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | GGATTAATTGACGAGGTCGG |
| Suggested primer 2: | AACGTAACTGGGAATCACGC |