| Insertion cassette: | CIB1 |
| Side of cassette: | 3' |
| Strand: | + |
| Strain: | LMJ.RY0402.080426 |
| Chromosome: | chromosome 2 |
| Location: | 1657476 |
| Confidence (%): | 73 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre02.g085600 | (1 of 2) IPR005645//IPR029058 - Serine hydrolase FSH // Alpha/Beta hydrolase fold | intron |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | CGCGGCGGACGAGAAGTTGCGAGGCCCTTG |
| Internal bar code: | TACCAGCCAGGCAGCGTGTGGG |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 643 |
| LEAP-Seq percent confirming: | 97.3597 |
| LEAP-Seq n confirming: | 295 |
| LEAP-Seq n nonconfirming: | 8 |
| LEAP-Seq n unique pos: | 4 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | ATCCACCCGTAGTCACAAGC |
| Suggested primer 2: | GAGCTGGAGAGGGTGAACAG |