| Insertion cassette: | CIB1 |
| Side of cassette: | 5' |
| Strand: | + |
| Strain: | LMJ.RY0402.080436 |
| Chromosome: | chromosome 10 |
| Location: | 4537284 |
| Confidence (%): | 73 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre10.g452200 | intron |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | GCTGCAGCGTCGATGCACCTGCACGTGAAT |
| Internal bar code: | CATTGGGTCGCGTAGAGAAAAT |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 414 |
| LEAP-Seq percent confirming: | 98.0347 |
| LEAP-Seq n confirming: | 1696 |
| LEAP-Seq n nonconfirming: | 34 |
| LEAP-Seq n unique pos: | 18 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | GAGATGAAAACAGCGCACAA |
| Suggested primer 2: | TCCACTTCCCACCTCTTGAC |