| Insertion cassette: | CIB1 |
| Side of cassette: | 5' |
| Strand: | - |
| Strain: | LMJ.RY0402.080462 |
| Chromosome: | chromosome_16 |
| Location: | 2218438 |
| Confidence (%): | 73 |
| Locus systematic id | Locus common name | Defline | Orientation | Feature |
|---|---|---|---|---|
| Cre16.g658700 | antisense | 5'UTR |
Insertion site details | |
| Flanking sequence (orientation from cassette outwards): | TTCTGGCACTACTAAATTTTCAGCACGCAG |
| Internal bar code: | AATGCCGCAACCGCTAGGATGG |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 528 |
| LEAP-Seq percent confirming: | 90.982 |
| LEAP-Seq n confirming: | 6235 |
| LEAP-Seq n nonconfirming: | 618 |
| LEAP-Seq n unique pos: | 6 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | GCCCAAGCTCTCACTTTGTC |
| Suggested primer 2: | CCGCTTCAGGTACAAAGAGG |