| Insertion cassette: | CIB1 |
| Side of cassette: | 3' |
| Strand: | + |
| Strain: | LMJ.RY0402.080462 |
| Chromosome: | chromosome 16 |
| Location: | 2219008 |
| Confidence (%): | 73 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre16.g658700 | FAP376 | (1 of 3) PF01661 - Macro domain (Macro); Flagellar Associated Protein 376 with ADP-ribose binding site | intron |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | GTTACGGCCACTGGGCCGGCCCTGGTGCAT |
| Internal bar code: | GCTAATGTACCTTTGCCGGGAT |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 611 |
| LEAP-Seq percent confirming: | 99.0941 |
| LEAP-Seq n confirming: | 9626 |
| LEAP-Seq n nonconfirming: | 88 |
| LEAP-Seq n unique pos: | 56 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | CGCTAGAGTCACAAATGGCA |
| Suggested primer 2: | AAGAAACGAGCAGGGCAGTA |