| Insertion cassette: | CIB1 |
| Side of cassette: | 3' |
| Strand: | + |
| Strain: | LMJ.RY0402.080463 |
| Chromosome: | chromosome 5 |
| Location: | 2966364 |
| Confidence (%): | 95 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre05.g238311 | 3'UTR |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | AGCCGTGATCGGGTCAATATGGAGCGATCA |
| Internal bar code: | GACGAAAGATGTTTCCGTCAGG |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 775 |
| LEAP-Seq percent confirming: | 94.157 |
| LEAP-Seq n confirming: | 6752 |
| LEAP-Seq n nonconfirming: | 419 |
| LEAP-Seq n unique pos: | 71 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | TTCCTGGCCAGATGATTAGG |
| Suggested primer 2: | CTCCAAGGTGGGTGTGACTT |