Insertion cassette: | CIB1 |
Side of cassette: | 5' |
Strand: | + |
Strain: | LMJ.RY0402.080524 |
Chromosome: | chromosome_2 |
Location: | 3519616 |
Confidence (%): | 73 |
Locus systematic id | Locus common name | Defline | Orientation | Feature |
---|---|---|---|---|
Cre02.g095126 | DPE2 | alpha-1,4-glucanotransferase | antisense | 3'UTR |
Insertion site details | |
Flanking sequence (orientation from cassette outwards): | CCCGCGTTCAGGCAGGCGCCGCCAATCGCC |
Internal bar code: | GTACATCACATAATGGACCGGT |
Confirmation - LEAP-Seq | |
LEAP-Seq distance: | 302 |
LEAP-Seq percent confirming: | 99.5336 |
LEAP-Seq n confirming: | 1494 |
LEAP-Seq n nonconfirming: | 7 |
LEAP-Seq n unique pos: | 3 |
Suggested primers for confirmation by PCR | |
Suggested primer 1: | GAGCATCTTTCATCCCCAAA |
Suggested primer 2: | CAGCGAGCTAGGGAATCAAC |