Insertion junction: LMJ.RY0402.080526_1

Overview

Insertion cassette:CIB1
Side of cassette:5'
Strand:-
Strain:LMJ.RY0402.080526
Chromosome:chromosome 14
Location:736952
Confidence (%):95
Locus systematic id Locus common name Defline Feature
Cre14.g612700 FAP50,DRC7 Nexin-dynein regulatory complex 7; (1 of 1) PF13415//PF13418//PF13855 - Galactose oxidase, central domain (Kelch_3) // Galactose oxidase, central domain (Kelch_4) // Leucine rich repeat (LRR_8) intron

Insertion site details

Expected flanking sequence (starting at cassette; orientation from cassette outwards): GTGAAGGGGGCCGCGTGAGGAGGCGGGCGG
Internal bar code:AGGTCCGTAGGGGGCCCCAGCG

Confirmation - LEAP-Seq

LEAP-Seq distance:207
LEAP-Seq percent confirming:99.6195
LEAP-Seq n confirming:5236
LEAP-Seq n nonconfirming:20
LEAP-Seq n unique pos:10

Suggested primers for confirmation by PCR

Suggested primer 1:GCGTGTGTGTGTGTGTGTGT
Suggested primer 2:TTGACGGTAGAAGGGGTCAC