| Insertion cassette: | CIB1 |
| Side of cassette: | 5' |
| Strand: | - |
| Strain: | LMJ.RY0402.080526 |
| Chromosome: | chromosome_14 |
| Location: | 736952 |
| Confidence (%): | 95 |
| Locus systematic id | Locus common name | Defline | Orientation | Feature |
|---|---|---|---|---|
| Cre14.g612700 | DRC7,FAP50 | Flagellar Associated Protein | sense | intron |
Insertion site details | |
| Flanking sequence (orientation from cassette outwards): | GTGAAGGGGGCCGCGTGAGGAGGCGGGCGG |
| Internal bar code: | AGGTCCGTAGGGGGCCCCAGCG |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 207 |
| LEAP-Seq percent confirming: | 99.6195 |
| LEAP-Seq n confirming: | 5236 |
| LEAP-Seq n nonconfirming: | 20 |
| LEAP-Seq n unique pos: | 10 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | GCGTGTGTGTGTGTGTGTGT |
| Suggested primer 2: | TTGACGGTAGAAGGGGTCAC |