| Insertion cassette: | CIB1 |
| Side of cassette: | 3' |
| Strand: | + |
| Strain: | LMJ.RY0402.080533 |
| Chromosome: | chromosome 2 |
| Location: | 4291377 |
| Confidence (%): | 95 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre02.g099055 | (1 of 18) PTHR19862:SF14 - WD REPEAT-CONTAINING PROTEIN 48 | CDS |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | TCCACCACAAACGCCAGCGCTGCCAGCAGC |
| Internal bar code: | ATCGCATTAGGCGCACAAAGTT |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 608 |
| LEAP-Seq percent confirming: | 85.0394 |
| LEAP-Seq n confirming: | 2592 |
| LEAP-Seq n nonconfirming: | 456 |
| LEAP-Seq n unique pos: | 34 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | TGACTTACCTCGACAGCGTG |
| Suggested primer 2: | CACACACACACACACACGGT |