| Insertion cassette: | CIB1 |
| Side of cassette: | 5' |
| Strand: | + |
| Strain: | LMJ.RY0402.080535 |
| Chromosome: | chromosome 9 |
| Location: | 199591 |
| Confidence (%): | 58 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre09.g386743 | (1 of 1) 2.1.1.305 - 8-demethyl-8-alpha-L-rhamnosyl tetracenomycin-C 2'-O-methyltransferase | 3'UTR |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | TCGATGCGACCGCAATGGCTAAGGGGCAAG |
| Internal bar code: | AACGGTTTGAATAGTGGAACAG |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 370 |
| LEAP-Seq percent confirming: | 97.9205 |
| LEAP-Seq n confirming: | 1601 |
| LEAP-Seq n nonconfirming: | 34 |
| LEAP-Seq n unique pos: | 5 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | CTACGCCAATGACCCGTACT |
| Suggested primer 2: | TGCACCCTACGGGACTTTAC |