| Insertion cassette: | CIB1 |
| Side of cassette: | 5' |
| Strand: | + |
| Strain: | LMJ.RY0402.080537 |
| Chromosome: | chromosome 16 |
| Location: | 2325695 |
| Confidence (%): | 58 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre16.g659500 | (1 of 3) 2.4.1.228 - Lactosylceramide 4-alpha-galactosyltransferase / Histo-blood group P(k) UDP-galactose | intron |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | GGTTTGCGCTGGTGAGCAGTATGCGGGCAC |
| Internal bar code: | TTTGTAAGCGTCCCAGAACTTG |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 309 |
| LEAP-Seq percent confirming: | 97.9452 |
| LEAP-Seq n confirming: | 143 |
| LEAP-Seq n nonconfirming: | 3 |
| LEAP-Seq n unique pos: | 3 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | TTGTTTCCAAGTGGTGGTGA |
| Suggested primer 2: | ACATCTTCCTGGTCGGTCTG |