| Insertion cassette: | CIB1 |
| Side of cassette: | 3' |
| Strand: | + |
| Strain: | LMJ.RY0402.080545 |
| Chromosome: | chromosome 12 |
| Location: | 7338502 |
| Confidence (%): | 58 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre12.g557250 | (1 of 15) 3.6.5.5 - Dynamin GTPase | intron |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | GTACACGCATGCGGCAATTCCAGATAGTAA |
| Internal bar code: | TTCGGCGTATGTCATAATTAGG |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 168 |
| LEAP-Seq percent confirming: | 99.2383 |
| LEAP-Seq n confirming: | 912 |
| LEAP-Seq n nonconfirming: | 7 |
| LEAP-Seq n unique pos: | 2 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | GGGTTTCGTTGCACAGTTTT |
| Suggested primer 2: | TAAAGATGCAGTGGTGCTGG |