Insertion junction: LMJ.RY0402.080547_1

Overview

Insertion cassette:CIB1
Side of cassette:5'
Strand:-
Strain:LMJ.RY0402.080547
Chromosome:chromosome 1
Location:2308868
Confidence (%):73
Locus systematic id Locus common name Defline Feature
Cre01.g012650 KCN9 (1 of 4) IPR000595//IPR002110//IPR005821//IPR018490//IPR020683 - Cyclic nucleotide-binding domain // Ankyrin repeat // Ion transport domain // Cyclic nucleotide-binding-like // Ankyrin repeat-containing domain; Voltage-gated potassium channel intron

Insertion site details

Expected flanking sequence (starting at cassette; orientation from cassette outwards): CCTTACTTGCGCATGTTTACACCACGCTTC
Internal bar code:GGACATCTCGTATCAGTACTG

Confirmation - LEAP-Seq

LEAP-Seq distance:650
LEAP-Seq percent confirming:98.801
LEAP-Seq n confirming:412
LEAP-Seq n nonconfirming:5
LEAP-Seq n unique pos:4

Suggested primers for confirmation by PCR

Suggested primer 1:ATGTGCACAAGGTGGTTCAA
Suggested primer 2:TGCTAGTGCTCCACCTTCCT