| Insertion cassette: | CIB1 |
| Side of cassette: | 3' |
| Strand: | + |
| Strain: | LMJ.RY0402.080586 |
| Chromosome: | chromosome 13 |
| Location: | 3427664 |
| Confidence (%): | 95 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre13.g587350 | intron |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | GTTTTCAAATGGCCCAAGCATCTTTGGCCC |
| Internal bar code: | TCCACACAGCGCGGAACTTGGG |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 308 |
| LEAP-Seq percent confirming: | 98.9508 |
| LEAP-Seq n confirming: | 3678 |
| LEAP-Seq n nonconfirming: | 39 |
| LEAP-Seq n unique pos: | 12 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | TTCTCGTGATCAACCCATCA |
| Suggested primer 2: | TCTGGATCTGCTATGGACCC |