Insertion junction: LMJ.RY0402.080594_2

Overview

Insertion cassette:CIB1
Side of cassette:5'
Strand:-
Strain:LMJ.RY0402.080594
Chromosome:chromosome 11
Location:3236250
Confidence (%):58
Locus systematic id Locus common name Defline Feature
Cre11.g479650 CHIP,CHIP1 (1 of 1) K09561 - STIP1 homology and U-box containing protein 1 (STUB1, CHIP); Carboxyl terminus of Hsc70-interacting protein intron

Insertion site details

Expected flanking sequence (starting at cassette; orientation from cassette outwards): TAATTACTCCCGCACCGAAGTCCACACCCA
Internal bar code:GGTGTAAAGGTCACACCGGATG

Confirmation - LEAP-Seq

LEAP-Seq distance:299
LEAP-Seq percent confirming:99.5449
LEAP-Seq n confirming:875
LEAP-Seq n nonconfirming:4
LEAP-Seq n unique pos:3

Suggested primers for confirmation by PCR

Suggested primer 1:GAAACTGCTGCCTCAACCTC
Suggested primer 2:TCCTATGCGAGAGAGGAGGA