| Insertion cassette: | CIB1 |
| Side of cassette: | 5' |
| Strand: | + |
| Strain: | LMJ.RY0402.080620 |
| Chromosome: | chromosome 14 |
| Location: | 1316701 |
| Confidence (%): | 95 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre14.g616850 | (1 of 7) PF00582 - Universal stress protein family (Usp) | intron |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | CGGAACTGAAATTCAGGAAACCGATAGTAT |
| Internal bar code: | GGGAGTGCATTATATCCGGCTC |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 604 |
| LEAP-Seq percent confirming: | 99.0566 |
| LEAP-Seq n confirming: | 105 |
| LEAP-Seq n nonconfirming: | 1 |
| LEAP-Seq n unique pos: | 2 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | CAGGAGCAGCAACAGCAGTA |
| Suggested primer 2: | TCAGCCAGAGCTCCTGTTTT |