| Insertion cassette: | CIB1 |
| Side of cassette: | 3' |
| Strand: | + |
| Strain: | LMJ.RY0402.080622 |
| Chromosome: | chromosome 10 |
| Location: | 6507074 |
| Confidence (%): | 58 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre10.g466550 | TF2H1 | General transcription factor IIH subunit 1; (1 of 1) IPR005607//IPR027079 - BSD domain // TFIIH subunit Tfb1/p62 | 3'UTR |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | AGCGCGCTTGGTTATGTGAGCGTACTAGCT |
| Internal bar code: | GCGTCTTCTGGGTAAGGAGGGG |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 372 |
| LEAP-Seq percent confirming: | 99.5152 |
| LEAP-Seq n confirming: | 821 |
| LEAP-Seq n nonconfirming: | 4 |
| LEAP-Seq n unique pos: | 5 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | CAGCCTGTCTCTCTTGACCC |
| Suggested primer 2: | TTGTAATCGATCAGCTTGCG |