Insertion junction: LMJ.RY0402.080643_1

Overview

Insertion cassette:CIB1
Side of cassette:5'
Strand:+
Strain:LMJ.RY0402.080643
Chromosome:chromosome 12
Location:3986414
Confidence (%):73
Locus systematic id Locus common name Defline Feature
Cre12.g516750 (1 of 55) PTHR23257//PTHR23257:SF394 - SERINE-THREONINE PROTEIN KINASE // SUBFAMILY NOT NAMED 3'UTR

Insertion site details

Expected flanking sequence (starting at cassette; orientation from cassette outwards): GACTCTTTCCGCAATCAGAGGTTGACGCGC
Internal bar code:AAGTTTCGCAACGCGGTTCGC

Confirmation - LEAP-Seq

LEAP-Seq distance:813
LEAP-Seq percent confirming:99.5724
LEAP-Seq n confirming:31435
LEAP-Seq n nonconfirming:135
LEAP-Seq n unique pos:20

Suggested primers for confirmation by PCR

Suggested primer 1:CCTATCCATGGAAATCCCCT
Suggested primer 2:GAAGTGATGCTGTCTGGCAA