| Insertion cassette: | CIB1 |
| Side of cassette: | 5' |
| Strand: | + |
| Strain: | LMJ.RY0402.080660 |
| Chromosome: | chromosome 6 |
| Location: | 5233205 |
| Confidence (%): | 73 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre06.g282200 | SNF7,VPS32 | Subunit of the ESCRT-III complex, SNF homolog | 5'UTR |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | CTACGGGGAAAGCGCAAGGGTAAGAAGAAT |
| Internal bar code: | CCTGAAAATCGGTTACTCCATA |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 539 |
| LEAP-Seq percent confirming: | 99.8798 |
| LEAP-Seq n confirming: | 831 |
| LEAP-Seq n nonconfirming: | 1 |
| LEAP-Seq n unique pos: | 4 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | CCCTCACCAGGTCAATGAGT |
| Suggested primer 2: | CTGCTGGATTGCAAAACTGA |