| Insertion cassette: | CIB1 |
| Side of cassette: | 5' |
| Strand: | - |
| Strain: | LMJ.RY0402.080670 |
| Chromosome: | chromosome 5 |
| Location: | 907712 |
| Confidence (%): | 95 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre05.g247300 | (1 of 1) PF13432//PF13489 - Tetratricopeptide repeat (TPR_16) // Methyltransferase domain (Methyltransf_23) | 5'UTR |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | CTGTATTAAGTGCTTGTACAACTGGTATTG |
| Internal bar code: | TCTTTCAATCCGTTGTCCTGAC |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 344 |
| LEAP-Seq percent confirming: | 99.6517 |
| LEAP-Seq n confirming: | 22316 |
| LEAP-Seq n nonconfirming: | 78 |
| LEAP-Seq n unique pos: | 12 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | CAGGTTGGAAGGAATGGAGA |
| Suggested primer 2: | CTCCTGTCACCTGCTGCATA |