| Insertion cassette: | CIB1 |
| Side of cassette: | 3' |
| Strand: | + |
| Strain: | LMJ.RY0402.080700 |
| Chromosome: | chromosome 8 |
| Location: | 28554 |
| Confidence (%): | 73 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre08.g358350 | OPR34,TDA1 | Translation factor for atpA | CDS |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | GGCGCTGGTGAGCATCAGCCAGCTGCGCTT |
| Internal bar code: | TACGGCACGGGAGACTAAGGGG |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 503 |
| LEAP-Seq percent confirming: | 97.2644 |
| LEAP-Seq n confirming: | 4800 |
| LEAP-Seq n nonconfirming: | 135 |
| LEAP-Seq n unique pos: | 18 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | GTGCTATGTTTCCTCGCCTC |
| Suggested primer 2: | CAGGGAAGCCATAGATTGGA |