Insertion cassette: | CIB1 |
Side of cassette: | 3' |
Strand: | + |
Strain: | LMJ.RY0402.080716 |
Chromosome: | chromosome 9 |
Location: | 546957 |
Confidence (%): | 95 |
Locus systematic id | Locus common name | Defline | Feature |
---|---|---|---|
Cre09.g403850 | (1 of 30) PTHR24349:SF79 - NITROGEN NETWORK KINASE 1 | 3'UTR |
Insertion site details | |
Expected flanking sequence (starting at cassette; orientation from cassette outwards): | CCGTTGGGCCGAACGGGAGGTAGGCGTGAC |
Internal bar code: | GCGCGAAGTCACAACTCAGAGC |
Confirmation - LEAP-Seq | |
LEAP-Seq distance: | 615 |
LEAP-Seq percent confirming: | 99.0149 |
LEAP-Seq n confirming: | 6131 |
LEAP-Seq n nonconfirming: | 61 |
LEAP-Seq n unique pos: | 27 |
Suggested primers for confirmation by PCR | |
Suggested primer 1: | GGGTTTCTCAGTGATTCCGA |
Suggested primer 2: | TGCAGTCACCCTTGACTCTG |