| Insertion cassette: | CIB1 |
| Side of cassette: | 5' |
| Strand: | - |
| Strain: | LMJ.RY0402.080719 |
| Chromosome: | chromosome 16 |
| Location: | 1992795 |
| Confidence (%): | 73 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre16.g656700 | KIN14A-2,KIN14-5,KIN14A2 | Kinesin motor protein; (1 of 1) PTHR24115//PTHR24115:SF464 - FAMILY NOT NAMED // KINESIN-1-RELATED | intron |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | GAGAGGTTAGGCTCTGGGGCGGGTTGTGCA |
| Internal bar code: | TGATGCGCTACATTTCCCTCAC |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 613 |
| LEAP-Seq percent confirming: | 99.8598 |
| LEAP-Seq n confirming: | 4274 |
| LEAP-Seq n nonconfirming: | 6 |
| LEAP-Seq n unique pos: | 6 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | ACATGTTTGCGCATTGGTAA |
| Suggested primer 2: | CACAACATTATCCAGGTGCG |