| Insertion cassette: | CIB1 |
| Side of cassette: | 5' |
| Strand: | + |
| Strain: | LMJ.RY0402.080742 |
| Chromosome: | chromosome 11 |
| Location: | 3563791 |
| Confidence (%): | 73 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre11.g481700 | SPL17 | Pre-mRNA splicing factor; (1 of 1) PTHR13161:SF15 - SPLICING FACTOR, SUPPRESSOR OF WHITE-APRICOT HOMOLOG | 3'UTR |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | TTCATGCATGGGTCCCAGCACCAAAAGCTG |
| Internal bar code: | GCTCTGGCAGTGGCATTCAAAG |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 367 |
| LEAP-Seq percent confirming: | 98.5611 |
| LEAP-Seq n confirming: | 1233 |
| LEAP-Seq n nonconfirming: | 18 |
| LEAP-Seq n unique pos: | 2 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | CTTCGGGCTAGGACTCACTG |
| Suggested primer 2: | ACGACGGTGGAAACAAAAAG |