| Insertion cassette: | CIB1 |
| Side of cassette: | 5' |
| Strand: | - |
| Strain: | LMJ.RY0402.080744 |
| Chromosome: | chromosome 12 |
| Location: | 7696996 |
| Confidence (%): | 58 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre12.g554100 | intron |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | TATATCCCACCCACGCACGTACGCACCGGT |
| Internal bar code: | GTGGGATTTCTAGCTCGAGGCT |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 510 |
| LEAP-Seq percent confirming: | 97.1112 |
| LEAP-Seq n confirming: | 13514 |
| LEAP-Seq n nonconfirming: | 402 |
| LEAP-Seq n unique pos: | 7 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | TAACGCCTTACTTGCCTGCT |
| Suggested primer 2: | CATCGGGATATTGTCTGCCT |