| Insertion cassette: | CIB1 |
| Side of cassette: | 3' |
| Strand: | + |
| Strain: | LMJ.RY0402.080748 |
| Chromosome: | chromosome 16 |
| Location: | 4574407 |
| Confidence (%): | 73 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre16.g688050 | (1 of 1) PTHR32091//PTHR32091:SF0 - FAMILY NOT NAMED // EUKARYOTIC INITIATION FACTOR 4B-RELATED | intron |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | GTCAGCCCTGTCTCGCCCCCAGCTCGTCTT |
| Internal bar code: | GCACCGGCCCGTATGTACCTAT |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 698 |
| LEAP-Seq percent confirming: | 97.2866 |
| LEAP-Seq n confirming: | 2940 |
| LEAP-Seq n nonconfirming: | 82 |
| LEAP-Seq n unique pos: | 15 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | AACACCAGGTCACCGAAGTC |
| Suggested primer 2: | CTTGTCTCCTTTCGTCTGGC |