Insertion junction: LMJ.RY0402.080752_1

Overview

Insertion cassette:CIB1
Side of cassette:3'
Strand:-
Strain:LMJ.RY0402.080752
Chromosome:chromosome 7
Location:1505858
Confidence (%):73
Locus systematic id Locus common name Defline Feature
Cre07.g324000 NUP160 Nucleoporin 160; (1 of 54) IPR011047 - Quinonprotein alcohol dehydrogenase-like superfamily 3'UTR

Insertion site details

Expected flanking sequence (starting at cassette; orientation from cassette outwards): GCGTGCTGTGCGCGTGTCAAACTTCCCCCC
Internal bar code:GGCTAGATGGCGGGCTCTGCCT

Confirmation - LEAP-Seq

LEAP-Seq distance:652
LEAP-Seq percent confirming:98.8255
LEAP-Seq n confirming:2356
LEAP-Seq n nonconfirming:28
LEAP-Seq n unique pos:17

Suggested primers for confirmation by PCR

Suggested primer 1:TCTGGGTCTCGGGTAATGTC
Suggested primer 2:GCACCAGCTTGACCTTCTTC